Proiectul HepApTech va furniza un nou aptamer pentru GP-73. Cel puțin doi noi aptasenzori electrochimici capabili de detectarea descentralizată a nivelurilor foarte scăzute de GP-73 vor fi realizați pe baza literaturii de specialitate și a unor teste „în laborator”. Aptamerul pentru GPC3 va fi integrat în sisteme de administrare bazate pe vezicule lipidice, respectiv nanoroboți . Dispozitivele de diagnostic și tratament vor fi testate pe probe reale.
Diseminarea rezultatelor s-a făcut prin publicarea de articole ştiinţifice (2 articole publicate în 2025), participare la congrese (11 în 2025), cu prezentări invitate (3 în 2025), cu prezentări de tip poster (6 în 2025) și prezentări orale (2 în 2025), după cum urmează:
1.5.1. Articole ştiinţifice publicate în 2025:
Irimes, M.-B., Pusta, A., Coker, D., Martian, P. C., Suciu, M., Pandrea, S.-L.; Tertis, M., Cristea, C., Oprean, R. (2025). Dual-target electrochemical aptasensor for the simultaneous detection of interleukin-6 and tumor necrosis factor-α in biological fluids. Sens. Bio-Sensing Res, 49, 100866. https://doi.org/10.1016/j.sbsr.2025.100866.
Casian, M., Manea, I., Hosu-Stancioiu, O., Lobo Castañón, M. J., de-los-Santos-Álvarez, N., & Cristea, C. (2025). Targeting hepatocellular carcinoma with aptamers: from biomarker detection to therapeutic applications. TrAC Trends in Analytical Chemistry, 191, 118346. https://doi.org/10.1016/j.trac.2025.118346.
1.5.2. Conferințe – Speaker invitat:
Cecilia Cristea, Magdolna Casian, Alexandra Pusta, Oana Hosu-Stancioiu, Mihaela Tertiș. New Insights into Aptamers: Unraveling Their Selection Processes and Exploring Their Role in Diagnosis and Therapy. 7th International Symposium on Advances in Pharmaceutical Analysis – APA 2025 Conference, 24th – 27th of August 2025, Ankara, Turkey.
Cecilia Cristea, Magdolna Casian, Oana Hosu Stancioiu, Mihaela Tertis, Maria-Bianca Irimes, Alexandra Pusta, Electrochemical Aptasensors for Healthcare Applications. From SELEX to Sensors: The Use of Aptamers for Healthcare Applications, 76th Annual Meeting of the International Society of Electrochemistry, 7th -12th of September , Mainz, Germany, 2025.
Maria-Bianca Irimes, Mihaela Tertis, Alexandra Pusta, Radu Oprean, Cecilia Cristea, A dual aptasensor for inflamatory cytokines detection in biologocal fluids, 5th European Biosensor Symposium, 26th – 29th October, Taragona, Spain, 2025.
1.5.3. Lucrări științifice de tip poster:
Magdolna Casian, Oana Hosu-Stancioiu, Ioana Manea, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. Aptamer selection using real-time quantitative PCR assisted SELEX technology for targeted therapy of hepatocellular carcinoma. Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca University Days, 8th – 12th of December 2025, Cluj-Napoca, Romania
Ioana Manea, Magdolna Casian, Oana Hosu-Stancioiu, Bogdan Feier, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. Molecular recognition strategy for early detection of hepatocellular carcinoma. Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca University Days, 8th – 12th of December 2025, Cluj-Napoca, Romania.
Alexandra Pusta, Maria-Bianca Irimes, Daniel Coker, Paul Cristian Martian, Maria Suciu, Stanca-Lucia Pandrea, Mihaela Tertis, Radu Oprean, Cecilia Cristea. Tracking Inflammation in Long-COVID: A Non-Invasive Device for Cytokine Detection Versus ELISA. Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca University Days, 8th – 12th of December 2025, Cluj-Napoca, Romania.
Alexandra Pusta, Maria-Bianca Irimes, Mihaela Tertis, Radu Oprean, Cecilia Cristea. A Flexible Electrochemical Aptasensor for Ultrasensitive Cortisol Detection in Biological Fluids. 5th Eurpoean Symposium on Biosensors, 26th – 29th October 2025, Tarragona, Spain.
Magdolna Casian, Oana Hosu-Stancioiu, Ioana Manea, Dimas Suárez, Natalia Díaz, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. Aptamer based point-of-care monitoring of antibiotic treatment: from in-lab selection to electrochemical aptasensor development. 35th Anniversary World Congress on Biosensors (Biosensors 2025), 19th – 22nd of May 2025, Lisbon, Portugal.
Magdolna Casian, Oana Hosu-Stancioiu, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. In vitro selection of glypican-3 specific aptamers for active targeted drug delivery applications in hepatocellular carcinoma. Aptamers 2025 International Conference, 14th – 15th of April 2025, Oxford, UK (online).
1.5.4. Lucrări științifice de tip prezentare orală:
Ioana Manea, Magdolna Casian, Oana Hosu-Stancioiu, Bogdan Feier, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. Les nouvelles outils analytiques dans le diagnostic du carcinome hepatocellulaire: les aptamers et les aptacapteurs. 4ᵉ édition du Symposium de la recherche scientifique francophone, 30th - 31st of October 2025, Iași, Romania.
Magdolna Casian, Oana Hosu-Stancioiu, Ioana Manea, Dimas Suárez, Natalia Díaz, María Jesús Lobo Castañón, Noemí de-los-Santos-Álvarez, Cecilia Cristea. High affinity aptamers for vancomycin monitoring in clinical samples by magnetic beads-based SELEX. 23rd International Symposium and Summer School on Bioanalysis, 7th – 15th of July 2025, Zagreb, Croatia.